Flowjo 10 how to crop x axis how to#
Additionally, you’ll see how to fix crooked horizons, and tweak image rotation by hand. We show you how to set the crop box location, size, and ratio, and how to apply cropping for each individual photo, or several images in a group. The graphs below compare the same data using different contouring thresholds. Watch the video and learn how to crop, level, and straighten images in various ways.
FlowJo tries to determine which parameter contains the DNA quantitation information if it chooses the wrong one, select the correct one from the X-axis popup menu. You might want to visit the page giving hints for Cell Cycle analysis with FlowJo. Show Outliers controls whether events falling outside of the lowest contour are drawn with dots–thus, combining the advantages of dot plots (revealing low-frequency data) with the best of contour plots (allow high-frequency data to be accurately displayed). FlowJo brings up a graph window that is specially designed for cell cycle analyses. Logarithmic plots distribute the contour levels logarithmically in height each contour line encloses twice as many events as the previous. Thus, it is by default, on.Ģ%, 5% and 10% plots have 50, 20, and 10 contour lines (respectively) that are distributed so that the same number of cells fall between each pair of contour lines. Smoothing does not alter the data nor does it distort it. These include the option of drawing “2%”, “5%”, “10%”, or “Logarithmic” contour plots, and “outliers” or no outliers.
There are several options available in drawing the plots (available by clicking the Options disclosure triangle in the Graph window). Combined with the display of outliers, this generates an extremely informative graphic that does not over-emphasize narrow peaks nor hide low-frequency information. All contouring algorithms have advantages and disadvantages, but probability contouring is probably the most informative. This algorithm generates, in general, graphs which are most accurately interpreted by our brains, in terms of relative frequencies of subpopulations. FlowJo draws only one type of contour plot, using equal probability contouring.